This test is useful for quantifying deficits in social behavior in transgenic animals exhibiting autistic traits as well as evaluating novel chemical entities for their effect on social behavior. a Mice were tested in three paradigms for anxiety-like behavior in the following sequence: elevated plus maze (EPM), lightdark box (LDB), and open field (OF). The time spent sniffing each pencil cup, the time spent in each chamber, and the number of entries into each chamber are presented. Experimental design of tests for affective and cognitive behavior after chronic constriction injury (CCI). The subject then encounters the first intruder as well as a second never-before-met intruder under another pencil cup in the “social novelty” session. The open field (76×57×35 cm) has a 48-square grid floor (6×8 squares, 9.5 cm/side): there is an overhead light illuminating the central squares (all but the 24 perimeter squares were considered central). After habituation to the empty box, the subject encounters a never-before-met intruder under one pencil cup and an empty pencil cup in the “sociability” session. Behavior in the open field is an index of exploration, anxiety, and motor behavior (Blizard et al. Testing occurs in three sessions within a three-chambered box with openings between the chambers. Based on these inclinations, the Three Chamber Test can help identify rodents with deficits in sociability and/or social novelty. Rodents normally prefer to spend more time with another rodent (sociability) and will investigate a novel intruder more so than a familiar one (social novelty). Coupled with Any-Maze behavioral tracking software for objective data collection and analysis Apparatus Dimensions 40 x 40 x 35 cm Any-Maze behavioral tracking software Version 5. The Three-Chamber test assesses cognition in the form of general sociability and interest in social novelty in rodent models of CNS disorders. Three-Chamber Sociability and Social Novelty Test
0 kommentar(er)
